CELLULAR UPTAKE AND RELATIVE EFFICIENCY IN CELL INACTIVATION BY PHOTOACTIVATED SULFONATED MESO-TETRAPHENYLPORPHINES

被引:69
作者
BERG, K
BOMMER, JC
WINKELMAN, JW
MOAN, J
机构
[1] PORPHYRIN PROD INC, LOGAN, UT 84321 USA
[2] HARVARD UNIV, BRIGHAM & WOMENS HOSP, SCH MED, BOSTON, MA 02115 USA
关键词
D O I
10.1111/j.1751-1097.1990.tb08681.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Abstract— The cellular uptake, relative fluorescence quantum yields and photosensitizing efficiencies of meso‐tetraphenylporphines sulfonated to different degrees (TPPSn) have been investigated using the human carcinoma cell line NHIK 3025. The efficiencies of these dyes in photoinactivation of cells were highly dependent on the number of sulfonate groups on the derivatives. These differences in phototoxicity were primarily due to different abilities to be taken up by cells, but were also dependent upon the cellular localization of the dyes. TPPS1 and TPPS2a were more efficiently taken up by the cells than TPPS2o and TPPS4. Plasma membrane associated TPPS4 was less efficient in cell inactivation per quantum of fluorescence emitted than intracellularly located dye. This was also to some extent the case for TPPS1 but not for TPPS2a and TPPS2o. The results presented here indicate that TPPS2a and TPPS1 are the most promising of the TPPSns for possible future use in photodynamic therapy. Copyright © 1990, Wiley Blackwell. All rights reserved
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页码:775 / 781
页数:7
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