CONVERSION OF AMMONIA OR UREA INTO ESSENTIAL AMINO-ACIDS, L-LEUCINE, L-VALINE, AND L-ISOLEUCINE USING ARTIFICIAL CELLS CONTAINING AN IMMOBILIZED MULTIENZYME SYSTEM AND DEXTRAN-NAD+ .4. MALATE-DEHYDROGENASE FOR COENZYME RECYCLING

被引：4

作者：

GU, KF ^{[1
]}

CHANG, TMS ^{[1
]}

机构：

[1] MCGILL UNIV,FAC MED,ARTIFICIAL CELLS & ORGANS RES CTR,3655 DRUMMOND ST,MONTREAL H3G 1Y6,QUEBEC,CANADA

来源：

JOURNAL OF MOLECULAR CATALYSIS | 1990年 / 62卷 / 03期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1016/0304-5102(90)85228-A

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Artificial cells containing malate dehydrogenase (EC 1.1.1.37), leucine dehydrogenase (EC 1.4.1.9), urease (EC 3.5.1.5), and dextran-NAD+ were prepared. This immobilized multienzyme system effectively converted ammonia or urea into essential amino acids, L-leucine, L-valine and L-isoleucine. L-malate acted as a cosubstrate for the regeneration of dextran-NADH. Greater concentrations of L-malate favored higher conversion ratios. The effects of ammonium salts and urea on the reaction rate were also studied. The reaction rates in ammonium salt solutions were 36.9-78.2% of those in urea solutions. © 1990.

引用

页码：331 / 339

页数：9

共 29 条

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