A SERUM-FREE, PARTLY DEFINED MEDIUM, PDM-805, FOR AXENIC CULTIVATION OF ENTAMOEBA-HISTOLYTICA SCHAUDINN, 1903 AND OTHER ENTAMOEBA

被引:17
作者
DIAMOND, LS
CUNNICK, CC
机构
[1] Laboratory of Parasitic Diseases, Niaid, National Institutes of Health, Bethesda, Maryland
来源
JOURNAL OF PROTOZOOLOGY | 1991年 / 38卷 / 03期
关键词
AMMONIUM MOIETY; AXENIC CULTIVATION; CASEIN PEPTONE DIALYSATE; ENTAMOEBA-BARRETI; ENTAMOEBA-INVADENS; ENTAMOEBA-TERRAPINAE; LIPOPROTEIN-CHOLESTEROL SOLUTION; NUTRITION;
D O I
10.1111/j.1550-7408.1991.tb04430.x
中图分类号
Q95 [动物学];
学科分类号
071002 ;
摘要
We describe the first serum-free, partly defined medium (PDM-805)for cultivating the human enteric pathogen, Entamoeba histolytica, and the reptilian amebae E. barreti, E. invadens, and E. terrapinae. PDM-805 was developed by the stepwise replacement of yeast extract, bovine serum, and a casein peptone digest in TYI-S-33, a medium widely used for the axenic cultivation of these parasites. The defined components include amino acids, carbohydrates, B vitamins, ascorbic acid, tocopherol, thioctic acid, nucleic acid precursors, trace metals, and phosphate buffers. The undefined components include a highly purified bovine serum albumin, a lipoprotein-cholesterol solution from bovine serum, and a dialyzable, autoclavable, water-soluble growth factor(s) having a molecular weight of less than 3,500 prepared from casein peptone. To date, studies on the growth requirements of E. histolytica, strain 200:NIH, show the following are essential for sustained multiplication of this ameba: iron, glucose, biotin, folic acid, niacinamide, pantothenate, pyridoxal, riboflavin, thiamine, cysteine, an ammonium moiety (in addition to that present in cysteine), bovine serum albumin, lipoprotein-cholesterol, and casein peptone dialysate.
引用
收藏
页码:211 / 216
页数:6
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