MOLECULAR-CLONING, STRUCTURE AND EXPRESSION ANALYSIS OF A FULL-LENGTH MOUSE-BRAIN GLUTAMATE-DEHYDROGENASE CDNA

被引：32

作者：

TZIMAGIORGIS, G

MOSCHONAS, NK

机构：

[1] FDN RES & TECHNOL,INST MOLEC BIOL & BIOTECHNOL,POB 1527,GR-71110 HERAKLION,GREECE

[2] UNIV CRETE,DEPT BIOL,HERAKLION,GREECE

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1991年 / 1089卷 / 02期

关键词：

GLUTAMATE DEHYDROGENASE; OLIVOPONTO-CEREBELLAR ATROPHY; CDNA CLONING; AMINO ACID SEQUENCE; SIGNAL PEPTIDE; GENE EXPRESSION; (MOUSE);

D O I：

10.1016/0167-4781(91)90017-G

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We isolated and analysed a full-length mouse brain glutamate dehydrogenase (GLUD) cDNA as a preliminary step to use the mouse model for the investigation of GLUD function in neurotransmission and neurodegeneration. GLUD coding sequences were found highly conserved among mouse, human and rat. Northern blots revealed two transcripts with different ratios in different mouse organs implying some mechanism of tissue-specific expression. In contrast to human, mouse GLUD gene family appears not to contain an intronless member.

引用

页码：250 / 253

页数：4

共 18 条

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