INDUCTION AND INTRACELLULAR-LOCALIZATION OF HSP-72 AFTER RENAL ISCHEMIA

被引:94
作者
VANWHY, SK [1 ]
HILDEBRANDT, F [1 ]
ARDITO, T [1 ]
MANN, AS [1 ]
SIEGEL, NJ [1 ]
KASHGARIAN, M [1 ]
机构
[1] YALE UNIV,SCH MED,DEPT PATHOL,NEW HAVEN,CT 06510
来源
AMERICAN JOURNAL OF PHYSIOLOGY | 1992年 / 263卷 / 05期
关键词
HEAT SHOCK PROTEIN 72; CELL POLARITY; ACUTE RENAL FAILURE;
D O I
10.1152/ajprenal.1992.263.5.F769
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
To determine whether heat shock proteins (HSPs) might be active in cellular recovery following transient ischemia, we examined rat kidneys for 70-kDa HSP (HSP-70) mRNA expression, protein elaboration, and intracellular localization after 45 min of renal ischemia and reflow of 15 min, 2, 6, and 24 h. Inducible HSP-70 mRNA is present at 15 min of reperfusion, peaks between 2 and 6 h, and falls by 24 h. Inducible 72-kDa HSP (HSP-72) protein accumulates progressively through 24 h and is found in both soluble and microsomal fractions following ischemia. Within proximal tubules, immunofluorescent localization of HSP-72 is restricted to the apical domain at 15 min, is dispersed through the cytoplasm in a vesicular pattern at 2 and 6 h, and has migrated away from the apical domain at 24 h. A portion of the vesicular HSP-72 is associated with lysosomes; no intranuclear HSP-72 is detected. The course of mRNA induction, protein elaboration, and HSP-72 localization coincides with previously described changes in proximal tubule morphology and polarity following sublethal ischemic injury. HSP-72 may be instrumental in cellular remodeling and restitution of epithelial polarity during recovery from ischemic renal injury.
引用
收藏
页码:F769 / F775
页数:7
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