EFFECT OF AGGREGATION ON THE HEMATOPORPHYRIN-SENSITIZED PRODUCTION OF SINGLET MOLECULAR-OXYGEN

被引:57
作者
TANIELIAN, C
HEINRICH, G
机构
[1] Laboratoire de Photochimie, Ecole Européenne des Hautes Etudes, Industries Chimiques de Strasbourg, Strasbourg, 67008
关键词
D O I
10.1111/j.1751-1097.1995.tb03950.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The hematoporphyrin-sensitized production of singlet molecular oxygen, O-2((1) Delta(g)), has been investigated in methanol and in aqueous solution. The quantum yield for formation of O-2((1) Delta(g)) (Phi(Delta)) has been measured by both steady-state (oxygen consumption) and time-resolved (near-infrared luminescence) methods. In methanol, both techniques indicate that Phi(Delta) = 0.76 and the value remains independent of sensitizer concentration over a wide range. This finding is consistent with the dye persisting in a monomeric form in methanol solution. In contrast, Phi(Delta) decreases markedly with increasing sensitizer concentration in water due to dimerization of the dye. Analysis of the steady-state data indicates Phi(Delta) values of 0.74 and 0.12, respectively, for monomer and dimer. It is further shown that the efficiency whereby quenching of the triplet state by O-2 results in generation of O-2((1) Delta(g)) is substantially lower for the dimer than for the corresponding monomer. Because monomer and dimer possess quite different absorption spectral profiles, the efficacy for photodynamic action with hematoporphyrin exhibits a pronounced wavelength dependence.
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页码:131 / 135
页数:5
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