THE SACB AND SACC GENES ENCODING LEVANSUCRASE AND SUCRASE FORM A GENE-CLUSTER IN ZYMOMONAS-MOBILIS

被引:21
作者
GUNASEKARAN, P
MUKUNDAN, G
KANNAN, R
VELMURUGAN, S
AITABDELKADER, N
ALVAREZMACARIE, E
BARATTI, J
机构
[1] DEPT CHIM,BIOCATALYSE GRP,URA1320,F-13288 MARSEILLE 9,FRANCE
[2] MADURAI KAMARAJ UNIV,SCH BIOL SCI,DEPT MICROBIAL TECHNOL,MADURAI 625021,TAMIL NADU,INDIA
关键词
D O I
10.1007/BF00129392
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The Zymomonas mobilis gene sacB that encodes the extracellular levansucrase was cloned and expressed in Escherichia coli. The gene product exhibited both sucrose hydrolysis activity and levan forming capability. Sub-cellular fractionation of E. coli carrying pLSS41 revealed that about 95% of the total sucrase activity was detected in the cytoplasmic fraction. The levansucrase gene was overexpressed (about hundred fold) in E. coli under T7 polymerase expression system. Nucleotide sequence analysis of this gene revealed an open reading frame of 1269 bp long coding for a protein of 423 amino acids with a molecular mass of 46.7 KDa. The deduced amino acid sequence was identical to the N-terminal amino acids of protein A51 of Z. mobilis ZM4. Therefore, the product of sacB is levansucrase. This is the first extracellular enzyme of Z. mobilis sequenced which does not possess a signal sequence. This gene is located 198 bp upstream of sacC gene encoding for the extracellular sucrase forming a gene cluster
引用
收藏
页码:635 / 642
页数:8
相关论文
共 33 条
[1]  
AITABDELKADER N, 1993, THESIS U AIX MARSEIL
[2]   ZYMOMONAS-MOBILIS - A BACTERIUM FOR ETHANOL-PRODUCTION [J].
BARATTI, JC ;
BULOCK, JD .
BIOTECHNOLOGY ADVANCES, 1986, 4 (01) :95-115
[3]   THE ZYMOMONAS-MOBILIS GLF, ZWF, EDD, AND GLK GENES FORM AN OPERON - LOCALIZATION OF THE PROMOTER AND IDENTIFICATION OF A CONSERVED SEQUENCE IN THE REGULATORY REGION [J].
BARNELL, WO ;
LIU, J ;
HESMAN, TL ;
ONEILL, MC ;
CONWAY, T .
JOURNAL OF BACTERIOLOGY, 1992, 174 (09) :2816-2823
[4]   MUTATIONAL ANALYSIS OF SEGMENTAL STABILIZATION OF TRANSCRIPTS FROM THE ZYMOMONAS-MOBILIS GAP-PGK OPERON [J].
BURCHHARDT, G ;
KESHAV, KF ;
YOMANO, L ;
INGRAM, LO .
JOURNAL OF BACTERIOLOGY, 1993, 175 (08) :2327-2333
[5]   MOLECULAR CHARACTERIZATION OF THE ZYMOMONAS-MOBILIS ENOLASE (ENO) GENE [J].
BURNETT, ME ;
LIU, J ;
CONWAY, T .
JOURNAL OF BACTERIOLOGY, 1992, 174 (20) :6548-6553
[6]   CONSTRUCTION OF A NEW VECTOR FOR THE EXPRESSION OF FOREIGN GENES IN ZYMOMONAS-MOBILIS [J].
BYUN, MOK ;
KAPER, JB ;
INGRAM, LO .
JOURNAL OF INDUSTRIAL MICROBIOLOGY, 1986, 1 (01) :9-15
[7]   POLYMERASE AND HYDROLASE ACTIVITIES OF BACILLUS-SUBTILIS LEVANSUCRASE CAN BE SEPARATELY MODULATED BY SITE-DIRECTED MUTAGENESIS [J].
CHAMBERT, R ;
PETITGLATRON, MF .
BIOCHEMICAL JOURNAL, 1991, 279 :35-41
[8]   LEVANSUCRASE OF BACILLUS-SUBTILIS - KINETIC AND THERMODYNAMIC ASPECTS OF TRANSFRUCTOSYLATION PROCESSES [J].
CHAMBERT, R ;
GONZYTREBOUL, G .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1976, 62 (01) :55-64
[9]   GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE GENE FROM ZYMOMONAS-MOBILIS - CLONING, SEQUENCING, AND IDENTIFICATION OF PROMOTER REGION [J].
CONWAY, T ;
SEWELL, GW ;
INGRAM, LO .
JOURNAL OF BACTERIOLOGY, 1987, 169 (12) :5653-5662
[10]   CLONING AND SEQUENCING OF THE ALCOHOL DEHYDROGENASE-II GENE FROM ZYMOMONAS-MOBILIS [J].
CONWAY, T ;
SEWELL, GW ;
OSMAN, YA ;
INGRAM, LO .
JOURNAL OF BACTERIOLOGY, 1987, 169 (06) :2591-2597