ANALYSIS OF DIENESTROL AND ITS DOSAGE FORMS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引:3
作者
BAILEY, LC
BAILEY, CA
机构
[1] Department of Pharmaceutical Chemistry, College of Pharmacy, Rutgers—The State University of New Jersey, Piscataway, New Jersey
关键词
Dienestrol—analysis by high‐performance liquid chromatography; in various dosage forms; Estrogens; synthetic—high‐performance liquid chromatographic analysis in various dosage forms; High‐performance liquid chromatography—analysis; dienestrol in various dosage forms;
D O I
10.1002/jps.2600680432
中图分类号
R914 [药物化学];
学科分类号
100701 ;
摘要
A high‐performance liquid chromatographic (HPLC) analysis is described for dienestrol as a drug substance and in cream, foam, and tablet dosage forms. After incorporation of the drug or dosage form into a solvent mixture containing an internal standard, biphenyl, an aliquot was chromatographed using a reversed‐phase medium, followed by UV spectrophotometric detection at 254 nm. The response of the chromatographic system was linear over a concentration range corresponding to 50–200% of the labeled amount of dienestrol. Satisfactory accuracy and precision were confirmed by analyzing cream by the standard addition method. The advantages of the HPLC method are its simplicity, speed, and sensitivity, which permit direct analysis of single‐dose quantities of dienestrol. Copyright © 1979 Wiley‐Liss, Inc., A Wiley Company
引用
收藏
页码:508 / 510
页数:3
相关论文
共 10 条
[1]   PRE-FILTER FOR REMOVING SUSPENDED SOLIDS FROM HPLC SAMPLES [J].
BAILEY, LC .
JOURNAL OF CHEMICAL EDUCATION, 1977, 54 (07) :428-428
[2]  
GRAHAM JH, 1972, J ASSOC OFF ANA CHEM, V55, P190
[3]   ACETONITRILE-DIATOMACEOUS EARTH COLUMN FOR SEPARATION OF STEROIDS AND OTHER COMPOUNDS [J].
GRAHAM, RE ;
KENNER, CT .
JOURNAL OF PHARMACEUTICAL SCIENCES, 1973, 62 (11) :1845-1849
[4]   IDENTIFICATION AND ESTIMATION OF DIETHYLSTILBESTROL AND DIENESTROL BY HIGH-SPEED LIQUID-CHROMATOGRAPHY [J].
HESSE, C ;
PIETRZIK, K ;
HOTZEL, D .
CHROMATOGRAPHIA, 1977, 10 (05) :256-261
[5]  
NEUNINGER H, 1972, SCI PHARM, V40, P12
[6]   IDENTIFICATION AND DETERMINATION OF SYNTHETIC ESTROGENS IN PHARMACEUTICALS BY HIGH-SPEED, REVERSED-PHASE PARTITION CHROMATOGRAPHY [J].
ROOS, RW .
JOURNAL OF PHARMACEUTICAL SCIENCES, 1974, 63 (04) :594-599
[7]  
SKOOG DA, 1976, FUNDAMENTALS ANAL CH, P588
[8]   DETERMINATION OF DIENESTROL IN PHARMACEUTICALS [J].
SUMMA, AF ;
GRAHAM, JH .
JOURNAL OF PHARMACEUTICAL SCIENCES, 1965, 54 (04) :612-+
[9]  
1975, US PHARMACOPEIA, P640
[10]  
1975, NATIONAL FORMULARY, P201