DETERMINATION OF TOTAL FREE AMINO-ACIDS WITH O-PHTHALALDEHYDE AND N-ACETYL-L-CYSTEINE

被引:11
作者
HERNANDEZ, MJM [1 ]
CAMANAS, RMV [1 ]
ALVAREZCOQUE, MCG [1 ]
机构
[1] UNIV VALENCIA, FAC CHEM, DEPT ANALYT CHEM, E-46100 BURJASSOT, SPAIN
关键词
D O I
10.1016/0026-265X(90)90057-C
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A spectrophotometric procedure is proposed for the determination of total free amino acids after reaction with o-phthalaldehyde and N-acetyl-l-cysteine, using isoleucine as the reference. The procedure was applied to the analysis of five samples of widely different composition. Recoveries were 98-105%. © 1990.
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页码:288 / 293
页数:6
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