BETA-ENDORPHIN CHIMERIC PEPTIDES - TRANSPORT THROUGH THE BLOOD-BRAIN-BARRIER INVIVO AND CLEAVAGE OF DISULFIDE LINKAGE BY BRAIN

被引:60
作者
PARDRIDGE, WM [1 ]
TRIGUERO, D [1 ]
BUCIAK, JL [1 ]
机构
[1] UNIV CALIF LOS ANGELES,SCH MED,BRAIN RES INST,LOS ANGELES,CA 90024
关键词
D O I
10.1210/endo-126-2-977
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Water soluble peptides are normally not transported through the brain capillary wall, i.e. the blood-brain barrier (BBB). Chimeric peptides may be transportable through the BBB and are formed by the covalent coupling of a nontransportable peptide, e.g. β-endorphin, to a transportable peptide vector, e.g. cationized albumin, using disulfide-based coupling reagents such as N-succinimidyl 3-[2-pyridyldithio(propionate)] (SPDP). The transcytosis of peptide into brain parenchyma, as opposed to vascular sequestration of blood-borne peptide, was quantified using an internal carotid artery perfusion/capillary depletion method. It is shown that [125I]β-endorphin is not transported through the BBB, but is rapidly cleaved to free [125I] tyrosine via capillary peptidase. Therefore, chimeric peptide was prepared using [126I] [D-Ala2]β-endorphin (DABE), owing to the resistance of this analogue to peptidase degradation. The [125I] DABE-cationized albumin chimeric peptide is shown to enter brain parenchyma at a rate comparable to that reported previously for unconjugated cationized albumin. When the [125I] DABE-cationized albumin chimeric peptide was incubated with rat brain homogenate at 37 C, the free [125I] DABE was liberated from the cationized albumin conjugate prior to its subsequent degradation into free [125I] tyrosine. Approximately 50% of the chimeric peptide was cleaved within 60 sec of incubation at 37 C. These studies demonstrate that 1) [125I] β -endorphin is not transported through the BBB in its unconjugated form, 2) a [125I] DABE-cationized albumin chimeric peptide is transported through the BBB into brain parenchyma at a rate comparable to the unconjugated cationized albumin, and 3) brain contains the necessary disulfide reductases for rapid cleavage of the chimeric peptide into free β -endorphin and this cleavage occurs before degradation of the [125I] DABE into [125I] tyrosine. © 1990 by The Endocrine Society.
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页码:977 / 984
页数:8
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