IAPP AMYLIN GENE TRANSCRIPTIONAL CONTROL REGION - EVIDENCE FOR NEGATIVE REGULATION

被引：27

作者：

MOSSELMAN, S

HOPPENER, JWM

DEWIT, L

SOELLER, W

LIPS, CJM

JANSZ, HS

机构：

[1] STATE UNIV UTRECHT,PHYSIOL CHEM LAB,UTRECHT,NETHERLANDS

[2] STATE UNIV UTRECHT,DEPT INTERNAL MED,UTRECHT,NETHERLANDS

[3] PFIZER INC,DEPT MOLEC GENET,GROTON,CT 06340

来源：

FEBS LETTERS | 1990年 / 271卷 / 1-2期

关键词：

Diabetes mellitus type 2; Human IAPP/amylin gene; Negative regulation; Pancreatic; β-cell; Promoter activity;

D O I：

10.1016/0014-5793(90)80365-P

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Aberrant expression of the islet amyloid polypeptide (IAPP) gene might be involved in the pathogenesis of nun-insulin-dependent diabetes mellitus (NIDDM). Here, we report that IAPP promoter-luciferase constructs revealed tissue-specific activity. This activity was not mediated by cAMP. Sequential 5' deletions of the IAPP promoter caused a progressive derepression of the IAPP gene promoter in IAPP-producing cells. Comparison of the nucleotide sequence of the IAPP promoter with that of the insulin promoter (both active in pancreatic β-cells) reveals two sequence elements of putative importance: an insulin enhancer-like sequence and an element which corresponds to a protected domain in rat insulin I gene promoter footprint experiments. © 1990.

引用

页码：33 / 36

页数：4

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[2]

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