THE SACCHAROMYCES-CEREVISIAE SEC65 GENE ENCODES A COMPONENT OF YEAST SIGNAL RECOGNITION PARTICLE WITH HOMOLOGY TO HUMAN SRP19

被引:90
作者
STIRLING, CJ
HEWITT, EW
机构
[1] Department of Biochemistry and Molecular Biology, Medical School, University of Manchester
关键词
D O I
10.1038/356534a0
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
TRANSLOCATION of proteins across the endoplasmic reticulum (ER) membrane represents the first step in the eukaryotic secretory pathway. In mammalian cells, the targeting of secretory and membrane protein precursors to the ER is mediated by signal recognition particle (SRP), a cytosolic ribonucleoprotein complex comprising a molecule of 7SL RNA and six polypeptide subunits (relative molecular masses 9, 14, 19, 54, 68 and 72K) 1. In Saccharomyces cerevisiae, a homologue of the 54K subunit (SRP54) 2,3 co-purifies with a small cytoplasmic RNA, scR1 (refs 4,5). Genetic data indicate that SRP54 and scR1 are involved in translocation in vivo, suggesting the existence of an SRP-like activity in yeast 5,6. Whether this activity requires additional components similar to those found in mammalian SRP is not known. We have recently reported a genetic selection that led to the isolation of a yeast mutant, sec65-1, which is conditionally defective in the insertion of integral membrane proteins into the ER7. Here we report the cloning and sequencing of the SEC65 gene, which encodes a 31.2K protein with significant sequence similarity to the 19K subunit of human SRP (SRP19) 8. We also report the cloning of a multicopy suppressor of sec65-1, and its identification as the previously defined SRP54 gene, providing genetic evidence for an interaction between these gene products in vivo.
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页码:534 / 537
页数:4
相关论文
共 20 条
[1]   ISOLATION OF A YEAST GENE, SRH1, THAT ENCODES A HOMOLOG OF THE 54K SUBUNIT OF MAMMALIAN SIGNAL RECOGNITION PARTICLE [J].
AMAYA, Y ;
NAKANO, A ;
ITO, K ;
MORI, M .
JOURNAL OF BIOCHEMISTRY, 1990, 107 (03) :457-463
[2]   SRH1 PROTEIN, THE YEAST HOMOLOG OF THE 54 KDA SUBUNIT OF SIGNAL RECOGNITION PARTICLE, IS INVOLVED IN ER TRANSLOCATION OF SECRETORY PROTEINS [J].
AMAYA, Y ;
NAKANO, A .
FEBS LETTERS, 1991, 283 (02) :325-328
[3]   STRUCTURE AND EXPRESSION OF THE URA5 GENE OF SACCHAROMYCES-CEREVISIAE [J].
DEMONTIGNY, J ;
BELARBI, A ;
HUBERT, JC ;
LACROUTE, F .
MOLECULAR & GENERAL GENETICS, 1989, 215 (03) :455-462
[4]   STRUCTURAL AND FUNCTIONAL DISSECTION OF SEC62P, A MEMBRANE-BOUND COMPONENT OF THE YEAST ENDOPLASMIC-RETICULUM PROTEIN IMPORT MACHINERY [J].
DESHAIES, RJ ;
SCHEKMAN, R .
MOLECULAR AND CELLULAR BIOLOGY, 1990, 10 (11) :6024-6035
[5]   THE MOST ABUNDANT SMALL CYTOPLASMIC RNA OF SACCHAROMYCES-CEREVISIAE HAS AN IMPORTANT FUNCTION REQUIRED FOR NORMAL-CELL GROWTH [J].
FELICI, F ;
CESARENI, G ;
HUGHES, JMX .
MOLECULAR AND CELLULAR BIOLOGY, 1989, 9 (08) :3260-3268
[6]   SEC65 GENE-PRODUCT IS A SUBUNIT OF THE YEAST SIGNAL RECOGNITION PARTICLE REQUIRED FOR ITS INTEGRITY [J].
HANN, BC ;
STIRLING, CJ ;
WALTER, P .
NATURE, 1992, 356 (6369) :532-533
[7]   SACCHAROMYCES-CEREVISIAE AND SCHIZOSACCHAROMYCES-POMBE CONTAIN A HOMOLOG TO THE 54-KD SUBUNIT OF THE SIGNAL RECOGNITION PARTICLE THAT IN S-CEREVISIAE IS ESSENTIAL FOR GROWTH [J].
HANN, BC ;
PORITZ, MA ;
WALTER, P .
JOURNAL OF CELL BIOLOGY, 1989, 109 (06) :3223-3230
[8]   THE SIGNAL RECOGNITION PARTICLE IN SACCHAROMYCES-CEREVISIAE [J].
HANN, BC ;
WALTER, P .
CELL, 1991, 67 (01) :131-144
[9]  
HENIKOFF S, 1987, METHOD ENZYMOL, V155, P156
[10]   YEAST ESCHERICHIA-COLI SHUTTLE VECTORS WITH MULTIPLE UNIQUE RESTRICTION SITES [J].
HILL, JE ;
MYERS, AM ;
KOERNER, TJ ;
TZAGOLOFF, A .
YEAST, 1986, 2 (03) :163-167