CHLORIDE CHANNELS OF HIGH CONDUCTANCE IN THE MICROVILLOUS MEMBRANE OF TERM HUMAN PLACENTA

被引:41
作者
BROWN, PD
GREENWOOD, SL
ROBINSON, J
BOYD, RDH
机构
[1] ST MARYS HOSP, MANCHESTER M13 0JH, LANCS, ENGLAND
[2] MANCHESTER EPITHELIAL RES CTR, MANCHESTER, ENGLAND
基金
英国惠康基金;
关键词
D O I
10.1016/S0143-4004(05)80253-X
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The patch clamp technique has been used to study ion channels in the undisturbed mircovillous membrane of the human placental syncytiotrophoblast. In villi from 55 placentae delivered by caesarean section, high resistance seals were achieved in approximately 30 per cent of attempts. Of these, a large conductance chloride channel was identified in seven inside-out and two ‘cell’ attached patches. The channel had the following properties: (a) a slope conductance of 313±9 pS, (b) the presence of subconductance states, (c) voltage dependency, being open predominantly between ±20 mV and inactivating at more extreme potentials and (d) inhibition by DIDS (4-acetamido-4′-diisothiocyanostilbene 2,2-disulphonic acid). These are characteristic features of ‘maxi’ chloride channels which have been identified in a variety of cell types (Gogelein, 1988). The role of the chloride channel in ion transport by or homeostasis of the syncytiotrophoblast has yet to be determined. © 1993, Baillière Tindall. All rights reserved.
引用
收藏
页码:103 / 115
页数:13
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