PROTOCOLS FOR INVITRO SPORULATION, ASCOSPORE RELEASE, SEXUAL MATING, AND FERTILITY IN CROSSES OF LEPTOSPHAERIA-MACULANS

被引：29

作者：

MENGISTU, A ^{[1
]}

RIMMER, RS ^{[1
]}

WILLIAMS, PH ^{[1
]}

机构：

[1] UNIV MANITOBA,DEPT PLANT SCI,WINNIPEG R3T 2N2,MANITOBA,CANADA

来源：

PLANT DISEASE | 1993年 / 77卷 / 05期

关键词：

BLACK LEG; BRASSICA DISEASE; PHOMA-LINGAM; SPORE STORAGE;

D O I：

10.1094/PD-77-0538

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Leptosphaeria maculans ascospores were produced in vitro by pairing single ascospore isolates on V8 juice agar. Incubation for 7 days at 24 C with continuous fluorescent lighting provided for intermingling of the developing fungal colonies. Production of pseudothecia and ascospores was stimulated by pouring a 20-ml layer of 2% water agar cooled to 55 C over a culture grown on V8 juice agar, then incubating the dishes for 4 wk at 16 C under black light with a 12-hr photoperiod. Ascospores were recoverable only within 48 hr after they matured. Release of ascospores was facilitated by placing a drop of 5% beta-glucuronidase over the asci, followed in 30-60 sec by two drops of sterile water. The number of pseduothecia varied significantly among the isolates tested. Crosses between isolates WA11 X WA30, WA22 X WA13, WA22 X WA30, WA32 X WA30, WA43 X WA40, and WA74 X WA30 had the highest number of pseudothecia and were the most fertile of the isolates tested.

引用

页码：538 / 540

页数：3

共 12 条

[1]

BOUDART G, 1981, THESIS U SCI TECHNOL

[2]

HILL CB, 1988, ADV PLANT PATHOL, V6, P169

[3] SEASONAL PATTERNS OF ASCOSPORE DISCHARGE BY LEPTOSPHAERIA-MACULANS IN RELATION TO BLACKLEG OF OILSEED RAPE [J].

MCGEE, DC ;

PETRIE, GA .

PHYTOPATHOLOGY, 1979, 69 (06) :586-589

[4] PATHOGENICITY GROUPING OF ISOLATES OF LEPTOSPHAERIA-MACULANS ON BRASSICA-NAPUS CULTIVARS AND THEIR DISEASE REACTION PROFILES ON RAPID-CYCLING BRASSICAS [J].

MENGISTU, A ;

RIMMER, SR ;

KOCH, E ;

WILLIAMS, PH .

PLANT DISEASE, 1991, 75 (12) :1279-1282

[5]

MILLER PM, 1955, PHYTOPATHOLOGY, V45, P461

[6] DIFFERENTIAL HOST-PARASITE INTERACTIONS BETWEEN OILSEED RAPE AND LEPTOSPHAERIA-MACULANS, THE CAUSAL FUNGUS OF STEM CANKER [J].

NEWMAN, PL .

PLANT PATHOLOGY, 1984, 33 (02) :205-210

[7] SEXUAL COMPATIBILITY OF ISOLATES OF THE RAPESEED BLACKLEG FUNGUS LEPTOSPHAERIA-MACULANS FROM CANADA, AUSTRALIA, AND ENGLAND [J].

PETRIE, GA ;

LEWIS, PA .

CANADIAN JOURNAL OF PLANT PATHOLOGY-REVUE CANADIENNE DE PHYTOPATHOLOGIE, 1985, 7 (03) :253-255

[8]

PUNITHALINGHAM E, 1972, DESCRIPTIONS PATHOGE

[9]

SMITH H. C., 1964, TRANS BRIT MYCOL SOC, V47, P159

[10]

Venn L., 1979, Australasian Plant Pathology, V8, P5, DOI 10.1071/APP9790005

← 1 2 →