SUPERCRITICAL FLUID CHROMATOGRAPHY COUPLED WITH THE SALMONELLA MICROSUSPENSION MUTAGENICITY ASSAY

被引：2

作者：

PARRISH, TD ^{[1
]}

FRANCIS, ES ^{[1
]}

BOOTH, GM ^{[1
]}

EATOUGH, DJ ^{[1
]}

LEE, ML ^{[1
]}

机构：

[1] BRIGHAM YOUNG UNIV,DEPT CHEM,PROVO,UT 84602

来源：

FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY | 1992年 / 344卷 / 10-11期

关键词：

D O I：

10.1007/BF00323740

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

On-line bioassay-directed fractionation of environmental samples is important because of the need to assign toxicological activity to certain classes of chemicals. Supercritical fluid chromatography (SFC) was used to fractionate a coal tar standard (SRM 1597) and these fractions were successfully directed into the Salmonella microsuspension mutagenicity assay. The reverse-mutation assay used in these experiments measures mutagenicity in Salmonella typhimurium TA 98 and was performed in a total volume of approximately 200 mul. Coupling of SFC with the microsuspension assay yielded quantitative recovery of the coal tar mutagenic activity, and the SFC chromatogram was compared to the mutagenicity profile to determine the activity of each fraction. This method of coupling SFC with bioassays may have broad applicability for fractionating various environmental samples and for identifying a wide range of biological activities in such samples.

引用

页码：442 / 446

页数：5

共 37 条

[1] METHODS FOR DETECTING CARCINOGENS AND MUTAGENS WITH SALMONELLA-MAMMALIAN-MICROSOME MUTAGENICITY TEST [J].