DETERMINATION OF FREE URINARY CATECHOLAMINES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH ELECTROCHEMICAL DETECTION

被引：16

作者：

VOLIN, P ^{[1
]}

机构：

[1] UNIV HELSINKI,DEPT CHEM,DIV ANALYT CHEM,SF-00014 HELSINKI,FINLAND

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1994年 / 655卷 / 01期

关键词：

D O I：

10.1016/0378-4347(94)00029-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The present paper compares a manual high-performance liquid chromatographic (HPLC) method with electrochemical detection (ED) with an on-line HPLC-ED method for the determination of free catecholamines (CAs) (norepinephrine, epinephrine, and dopamine) in urine. The manual method involves a two-stage extraction procedure with home-packed columns filled with weakly acidic cation-exchange resin followed by adsorption onto alumina while the on-line method uses a strong cation-exchange polymer as precolumn material which allows the simultaneous determination of free CAs and metanephrines (MNs) in urine. Reference control urine samples analysed with both methods gave levels of CAs within the acceptable reference ranges. Dihydroxybenzylamine was used as an internal standard in both methods and the separation was performed by ion-pairing reversed-phase HPLC.

引用

页码：121 / 126

页数：6

共 14 条

[1]

SIMULTANEOUS DETERMINATION OF CATECHOLAMINES AND METANEPHRINES IN URINE BY HPLC WITH FLUOROMETRIC DETECTION [J].

ABELING, NG ;

VANGENNIP, AH ;

OVERMARS, H ;

VOUTE, PA .

CLINICA CHIMICA ACTA, 1984, 137 (02) :211-226

[2]

ANTON AH, 1962, J PHARMACOL EXP THER, V138, P360

[3]

SIMULTANEOUS QUANTITATION OF CATECHOLAMINES AND O-METHYLATED METABOLITES IN URINE BY ISOCRATIC ION-PAIRING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH AMPEROMETRIC DETECTION [J].

CHAN, YPM ;

SIU, TSS .

JOURNAL OF CHROMATOGRAPHY, 1988, 459 :251-260

[4]

URINARY EXCRETION OF CATECHOLAMINES AND THEIR METABOLITES IN PHEOCHROMOCYTOMA [J].

CROUT, JR ;

PISANO, JJ ;

SJOERDSMA, A .

AMERICAN HEART JOURNAL, 1961, 61 (03) :375-&

[5]

URINARY FREE CATECHOLAMINES - DIAGNOSTIC APPLICATION OF AN HPLC TECHNIQUE TO THE INVESTIGATION OF NEURAL CREST TUMORS [J].

DAVIDSON, DF .

ANNALS OF CLINICAL BIOCHEMISTRY, 1987, 24 :494-499

[6]

AN AUTOMATED-METHOD FOR THE ANALYSIS OF URINARY FREE CATECHOLAMINES USING ASTED AND HIGH-PRESSURE LIQUID-CHROMATOGRAPHY [J].

GREEN, B ;

COOPER, JDH ;

TURNELL, DC .

ANNALS OF CLINICAL BIOCHEMISTRY, 1989, 26 :361-367

[7]

KAGEDAL B, 1988, J CHROMATOGR-BIOMED, V429, P177, DOI 10.1016/S0378-4347(00)83871-2

[8]

KOPIN IJ, 1985, PHARMACOL REV, V37, P333

[9]

EFFECT OF DIET ON URINARY-EXCRETION OF UNCONJUGATED CATECHOLAMINES AND SOME OF THEIR METABOLITES IN HEALTHY CONTROL SUBJECTS AND DEPRESSED-PATIENTS [J].

MOLEMAN, P .

CLINICA CHIMICA ACTA, 1990, 189 (01) :19-24

[10]

MEASUREMENT OF CATECHOLAMINES, THEIR PRECURSOR AND METABOLITES IN HUMAN-URINE AND PLASMA BY SOLID-PHASE EXTRACTION FOLLOWED BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH FLUORESCENCE DERIVATIZATION [J].

NOHTA, H ;

YAMAGUCHI, E ;

OHKURA, Y ;

WATANABE, H .

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1989, 493 (01) :15-26

← 1 2 →