ENANTIOMERIC SEPARATION BY MICELLAR ELECTROKINETIC CHROMATOGRAPHY USING SAPONINS

Glycyrrhizic acid (GRA) and beta-escin were employed in micellar electrokinetic chromatography (MEKC) for enantiomeric separation. Both GRA and beta-escin are saponins and natural chiral surfactants, A mixed micelle consisting of GRA, octyl-beta-D-glucoside, and sodium dodecyl sulfate (SDS) was successfully used for enantiomeric separation of dansylated DL-amino acids. Beta-Escin was also employed to prepare a mixed micelle with SDS and the mixed micelle was capable of separating phenylthiohydantoin-DL-amino acids under acidic conditions.