FLOW CYTOMETRY OF HUMAN-SPERMATOZOA

被引：51

作者：

OTTO, FJ

HACKER, U

ZANTE, J

SCHUMANN, J

GOHDE, W

MEISTRICH, ML

机构：

[1] UNIV MUNSTER,FACHKLIN HORNHEIDE,EXPTL TUMORFORSCH ABT,D-4400 MUNSTER,FED REP GER

[2] UNIV TEXAS,MD ANDERSON HOSP & TUMOR INST,CTR CANC,EXPTL RADIOTHERAPY SECT,HOUSTON,TX 77030

来源：

HISTOCHEMISTRY | 1979年 / 61卷 / 03期

关键词：

D O I：

10.1007/BF00508445

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Methods are given for the preparation and staining of human spermatozoa for flow cytometric DNA measurements. Using agents for the reductive cleavage of disulfide crosslinks and suitable proteolytic enzymes and effective decondensation of the sperm chromatin and a DNA-proportional uptake of fluorochromes is achieved. Thus reliable and precise measurements of the relative DNA content of human spermatozoa are possible and the two subpopulations of haploid spermatozoa can be distinguished according to the difference in their DNA content. © 1979 Springer-Verlag.

引用

页码：249 / 254

页数：6

共 14 条

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