PERMEATION PROPERTIES OF A NONSELECTIVE CATION CHANNEL IN HUMAN VASCULAR ENDOTHELIAL-CELLS

Endothelial cells obtained from human umbilical chord have been studied by the patch clamp method. An ion channel is described that is activated by μM concentrations of histamine and shows a slow run-down in cell-attached patches. After excision, channel activity quickly runs down to zero open probability. In symmetrical potassium concentrations (140 mM K in the bath and the pipette), the single channel conductance is 28±2 pS and the reversal potential is 0.3±0.8 mV (mean ± SEM, n=4). With 140 mM Na in the pipette, the conductance is 26±2 pS. A reversal potential of -1.5±0.9 mV (n=7) was measured. With 60 mM Ca and 70 mM Na in the pipette, 140 mM K in the bath, the reversal potential was -11±3 mV, the single channel conductance 16±3 pS (n=5). The single channel conductance in 110 mM Ca (pipette) and 140 mM K (bath) is 8±2 pS and the reversal potential is -18±6 mV (n=3). From analysis of the reversal potentials, a permeation ratio of K:Na:Ca=1:0.9:0.2 was calculated. This ligand-gated non-selective cation channel in human endothelial cells is Ca permeable and could induce a sustained agonist mediated Ca influx. © 1990 Springer-Verlag.