OCCURRENCE OF FREE CERAMIDES IN BACTEROIDES-FRAGILIS NCTC-9343

被引:35
作者
MIYAGAWA, E
AZUMA, R
SUTO, T
YANO, I
机构
[1] NATL INST ANIM HLTH,HOKKAIDO BRANCH LAB,SAPPORO,HOKKAIDO 06101,JAPAN
[2] OSAKA CITY UNIV,SCH MED,DEPT BACTERIOL,ABENO KU,OSAKA 545,JAPAN
关键词
D O I
10.1093/oxfordjournals.jbchem.a132528
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The occurrence of free ceramides at high concentrations was demonstrated in the chloroform-methanol extractable lipids of Bacteroides fragilis NCTC 9343. The long-chain bases were isolated from the free ceramides and identified as branched and normal saturated dihydroxy bases with carbon chains consisting of 17, 18, and 19 atoms. The major fatty acid was 3-hydroxy 15-methylhexadecanoic acid.The major molecular species of the ceramides were identified by gas chromatography-mass spectometry and gas chromatography of the cleaved products as LCB-d-iso17: 0-3-OH iso17: 0 FA, LCB-d-anteiso17: 0-3-OH iso17: 0 FA, LCB-d-iso18: 0-3-OH iso17: 0 FA, and LCB-d-anteisol9: 0-3-OH iso17: 0 FA. © 1979 by The Journal of Biochemistry.
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页码:311 / 320
页数:10
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