HUMAN ZN-ALPHA-2-GLYCOPROTEIN CDNA CLONING AND EXPRESSION ANALYSIS IN BENIGN AND MALIGNANT BREAST TISSUES

被引：49

作者：

FREIJE, JP ^{[1
]}

FUEYO, A ^{[1
]}

URIA, J ^{[1
]}

LOPEZOTIN, C ^{[1
]}

机构：

[1] UNIV OVIEDO, FAC MED, DEPT BIOL FUNC, E-33006 OVIEDO, SPAIN

来源：

FEBS LETTERS | 1991年 / 290卷 / 1-2期

关键词：

ZN-ALPHA-2-GLYCOPROTEIN; BREAST CYSTIC DISEASE; BREAST CANCER;

D O I：

10.1016/0014-5793(91)81271-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two cDNA clones coding for Zn-alpha-2-glycoprotein (Zn-alpha-2-gp) have been isolated from a human breast library and their nucleotide sequences determined. The deduced amino acid sequence contains the coding information for a hydrophobic signal peptide and the 278 residues of the mature protein. Comparison of this sequence with that from the protein purified from plasma reveals four differences: two amino acid changes (Gln-67 and Glu-222) and insertion of two residues (Ile-75 and Phe-76). Northern-blot analysis showed that the Zn-alpha-2-gp gene is expressed in liver and normal breast, but not in placenta, ovary and thyroid. A comparative analysis in mammary tissues from women with different diseases revealed enhanced expression of Zn-alpha-2-gp gene in benign breast lesions and a variable expression level in breast cancers.

引用

页码：247 / 249

页数：3

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