CLONING AND NUCLEOTIDE-SEQUENCE OF THE FABD GENE ENCODING MALONYL COENZYME A-ACYL CARRIER PROTEIN TRANSACYLASE OF ESCHERICHIA-COLI

被引：36

作者：

MAGNUSON, K

OH, W

LARSON, TJ

CRONAN, JE

机构：

[1] UNIV ILLINOIS,DEPT MICROBIOL,131 BURRILL HALL,407 S GOODWIN AVE,URBANA,IL 61801

[2] UNIV ILLINOIS,DEPT BIOCHEM,URBANA,IL 61801

[3] VIRGINIA POLYTECH INST & STATE UNIV,DEPT BIOCHEM & NUTR,BLACKSBURG,VA 24061

来源：

FEBS LETTERS | 1992年 / 299卷 / 03期

关键词：

MALONYL TRANSACYLASE; MALONYL-COA; ACYL TRANSFERASE; FATTY ACID BIOSYNTHESIS;

D O I：

10.1016/0014-5793(92)80128-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the cloning and nucleotide sequence of the gene encoding malonyl coenzyme A-acyl carrier protein transacylase of Escherichia coli. Malonyl transacylase has been overexpressed 155-fold compared to a wild-type strain. Overexpression of this enzyme alters the fatty acid composition of a wild-type E. coli strain; increased amounts of cis-vaccenate are incorporated into the membrane phospholipids.

引用

页码：262 / 266

页数：5

共 23 条

[1] THE MULTIFUNCTIONAL 6-METHYLSALICYLIC ACID SYNTHASE GENE OF PENICILLIUM-PATULUM - ITS GENE STRUCTURE RELATIVE TO THAT OF OTHER POLYKETIDE SYNTHASES [J].