INVITRO INFECTION OF HUMAN BONE-MARROW BY FELINE LEUKEMIA VIRUSES

被引：9

作者：

MORGAN, RA ^{[1
]}

DORNSIFE, RE ^{[1
]}

ANDERSON, WF ^{[1
]}

HOOVER, EA ^{[1
]}

机构：

[1] COLORADO STATE UNIV,DEPT PATHOL,FT COLLINS,CO 80523

来源：

VIROLOGY | 1993年 / 193卷 / 01期

关键词：

D O I：

10.1006/viro.1993.1141

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Four subgroup feline leukemia viruses (FeLV) were tested for their ability to infect primary cultures of human bone marrow in vitro. Using three sequential exposures to both concentrated and unconcentrated virus, FeLV infection of human bone marrow was documented by indirect immunofluorescence, ELISA, and polymerase chain reaction (PCR) assays. Based on comparison to known standards, it was estimated that between 5 and 10% of the bulk human marrow culture could be infected by FeLV. Further evidence for FeLV reverse transcription, integration, and expression was obtained using specific PCR assays. © 1993 Academic Press, Inc.

引用

页码：439 / 442

页数：4

共 21 条

[1] A MAJORITY OF MICE SHOW LONG-TERM EXPRESSION OF A HUMAN BETA-GLOBIN GENE AFTER RETROVIRUS TRANSFER INTO HEMATOPOIETIC STEM-CELLS [J].