SHORT-COLUMN GRADIENT ELUTION HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY FOR THE RAPID-DETERMINATION OF 7-ETHOXYCOUMARIN METABOLITES IN LIVER-SLICE INCUBATES

被引：9

作者：

HILLER, DL ^{[1
]}

COLE, RO ^{[1
]}

机构：

[1] PFIZER INC,CENT RES,DEPT DRUG METAB,GROTON,CT 06340

来源：

ANALYTICAL BIOCHEMISTRY | 1995年 / 227卷 / 01期

关键词：

D O I：

10.1006/abio.1995.1278

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An improved HPLC method for the analysis of 7-ethoxycoumarin and its metabolites in liver-slice incubation media is presented. The assay is based on short-column gradient elution which permits baseline resolution of the parent drug and three metabolites with a fourfold reduction in analysis time relative to existing HPLC methods. Total assay run time is 10 min. Data establishing method performance are presented which show intra- and interassay precision and accuracy to be within 13 and 7%, respectively, for separately prepared quality control samples. (C) 1995 Academic Press, Inc.

引用

页码：251 / 254

页数：4

共 5 条

[1] LIVER SLICES IN DYNAMIC ORGAN-CULTURE .2. AN INVITRO CELLULAR TECHNIQUE FOR THE STUDY OF INTEGRATED-DRUG METABOLISM USING HUMAN TISSUE [J].