EFFECTS OF APRAMYCIN, A NOVEL AMINOGLYCOSIDE ANTIBIOTIC ON BACTERIAL PROTEIN-SYNTHESIS

1. The novel aminoglycoside antibiotic apramycin is shown to be a potent inhibitor of protein synthesis in bacteria both invivo and invitro. 2. In cell‐free systems from Escherichia coli programmed with poly(U), apramycin induces translation errors, as assayed by incorporation of leucine, isoleucine and serine, although this effect occurs only to a limited extent. 3. Apramycin inhibits the translocation step of protein synthesis both invivo, in protoplasts of Bacillus megaterium, and invitro, in cell‐free systems from E. coli. It is proposed that this is the primary inhibitory effect of the drug. Copyright © 1979, Wiley Blackwell. All rights reserved