REVERSIBLE ADP-RIBOSYLATION OF THE 78-KDA GLUCOSE-REGULATED PROTEIN

被引：44

作者：

LENO, GH

LEDFORD, BE

机构：

[1] MED UNIV S CAROLINA,DEPT BIOCHEM & MOLEC BIOL,171 ASHLEY AVE,CHARLESTON,SC 29425

[2] MED UNIV S CAROLINA,PATHOBIOL PROGRAM,CHARLESTON,SC 29425

[3] MED UNIV S CAROLINA,MOLEC & CELLULAR BIOL PROGRAM,CHARLESTON,SC 29425

来源：

FEBS LETTERS | 1990年 / 276卷 / 1-2期

关键词：

ADP-RIBOSYLATION; GRP78/BIP; TUNICAMYCIN; AMINO ACID STARVATION; NUTRITIONAL STRESS;

D O I：

10.1016/0014-5793(90)80499-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Starvation of Mouse hepatoma cells for essential amino acids or glucose results in the mono-ADP-ribosylation of the 78 kDa glucose-regulated protein, GRP78. Here we show that the ADP-ribosylated and non-ADP-ribosylated forms of GRP78 are interconvertible during tryptophan starvation and refeeding. In addition, the ADP-ribosylation of GRP78 was shown to be reversible even during nutritional stress. The overexpressed pool of non-ADP-ribosylated GRP78 synthesized during tunicamycin treatment was available for ADP-ribosylation during subsequent amino acid starvation, especially in the absence of tunicamycin. The reversible ADP-ribosylation of GRP78 could be part of a metabolic control mechanism in operation during nutritional stress.

引用

收藏

页码：29 / 33

页数：5

相关论文

共 30 条

[1] POSTTRANSLATIONAL ASSOCIATION OF IMMUNOGLOBULIN HEAVY-CHAIN BINDING-PROTEIN WITH NASCENT HEAVY-CHAINS IN NONSECRETING AND SECRETING HYBRIDOMAS [J].

BOLE, DG ;

HENDERSHOT, LM ;

KEARNEY, JF .

JOURNAL OF CELL BIOLOGY, 1986, 102 (05) :1558-1566

[2] ADP-RIBOSYLATION OF THE MR 83,000 STRESS-INDUCIBLE AND GLUCOSE-REGULATED PROTEIN IN AVIAN AND MAMMALIAN-CELLS - MODULATION BY HEAT-SHOCK AND GLUCOSE STARVATION [J].

CARLSSON, L ;

LAZARIDES, E .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (15) :4664-4668

[3] FLUOROGRAPHIC DETECTION OF RADIOACTIVITY IN POLYACRYLAMIDE GELS WITH THE WATER-SOLUBLE FLUOR, SODIUM-SALICYLATE [J].

CHAMBERLAIN, JP .

ANALYTICAL BIOCHEMISTRY, 1979, 98 (01) :132-135

[4]

Collier RJ, 1982, ADP RIBOSYLATION REA, P575

[5] ENHANCEMENT OF POLYACRYLAMIDE-GEL SLICE DISSOLUTION IN HYDROGEN-PEROXIDE BY CUPRIC SULFATE [J].

DONATO, H ;

DOIG, MT ;

PRIEST, DG .

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, 1988, 15 (06) :331-335

[6] MOLECULAR CHAPERONES - PROTEINS ESSENTIAL FOR THE BIOGENESIS OF SOME MACROMOLECULAR STRUCTURES [J].

ELLIS, RJ ;

HEMMINGSEN, SM .

TRENDS IN BIOCHEMICAL SCIENCES, 1989, 14 (08) :339-342

[7] ENDOGENOUS ADP-RIBOSYLATION OF ELONGATION FACTOR-II IN POLYOMA VIRUS-TRANSFORMED BABY HAMSTER-KIDNEY CELLS [J].

FENDRICK, JL ;

IGLEWSKI, WJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (02) :554-557

[8] REVERSIBLE ADP-RIBOSYLATION IS DEMONSTRATED TO BE A REGULATORY MECHANISM IN PROKARYOTES BY HETEROLOGOUS EXPRESSION [J].

FU, H ;

BURRIS, RH ;

ROBERTS, GP .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (05) :1720-1724

[9] EXPRESSION OF WILD-TYPE AND MUTANT FORMS OF INFLUENZA HEMAGGLUTININ - THE ROLE OF FOLDING IN INTRACELLULAR-TRANSPORT [J].

GETHING, MJ ;

MCCAMMON, K ;

SAMBROOK, J .

CELL, 1986, 46 (06) :939-950

[10] IDENTITY OF THE IMMUNOGLOBULIN HEAVY-CHAIN-BINDING PROTEIN WITH THE 78,000-DALTON GLUCOSE-REGULATED PROTEIN AND THE ROLE OF POSTTRANSLATIONAL MODIFICATIONS IN ITS BINDING FUNCTION [J].

HENDERSHOT, LM ;

TING, J ;

LEE, AS .

MOLECULAR AND CELLULAR BIOLOGY, 1988, 8 (10) :4250-4256