A DBL-HOMOLOGOUS REGION OF THE YEAST CLS4/CDC24 GENE-PRODUCT IS IMPORTANT FOR CA2+-MODULATED BUD ASSEMBLY

被引：31

作者：

MIYAMOTO, S ^{[1
]}

OHYA, Y ^{[1
]}

SANO, Y ^{[1
]}

SAKAGUCHI, S ^{[1
]}

IIDA, H ^{[1
]}

ANRAKU, Y ^{[1
]}

机构：

[1] NATL INST BASIC BIOL,DIV CELL PROLIFERAT,OKAZAKI,AICHI 444,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 181卷 / 02期

关键词：

D O I：

10.1016/0006-291X(91)91233-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The CLS4 CDC24 is essential for the budding process of the yeast Saccharomyces cerevisiae. Disruption of the CLS4 CDC24 gene is lethal, and expression of the CLS4 product under the control of the GAL1 promoter is sufficient for cellular growth. The CLS4 product is detected in yeast cell lysate with an apparent molecular mass of 93 kD (854 amino acid residues) and shows homology with the human DBL oncogene product. Temperature-sensitive cdc24-1 mutation is located in the N-terminal portion of the protein whereas Ca2+-sensitive cls4-1 mutation is present after the DBL-homologous region (amino acid residues 281-518) near the putative Ca2+-binding site. Mutations within the DBL-homologous region are responsible for the Ca2+-sensitive phenotype. Thus the CLS4 gene product seems to have several functional domains within the molecule essential for bud assembly. © 1991.

引用

页码：604 / 610

页数：7

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