CHARACTERIZATION OF 10 MARKER CHROMOSOMES IN A PROSTATIC-CANCER CELL-LINE BY INSITU HYBRIDIZATION

被引：19

作者：

BROTHMAN, AR

PATEL, AM

机构：

[1] EASTERN VIRGINIA MED SCH,DEPT PEDIAT,NORFOLK,VA 23501

[2] EASTERN VIRGINIA MED SCH,DEPT MICROBIOL IMMUNOL,NORFOLK,VA 23501

来源：

CYTOGENETICS AND CELL GENETICS | 1992年 / 60卷 / 01期

关键词：

D O I：

10.1159/000133283

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Marker chromosomes contain potentially valuable information about breakpoints in cancer. However, routine banding procedures, by themselves, provide only limited information about the identity of marker chromosomes. In this study, the use of fluorescence in situ hybridization (FISH) with chromosome-specific centromeric probes and whole-chromosome-specific DNA libraries greatly enhanced the identification of 10 marker chromosomes in the primary prostatic cancer cell line PPC-1. Centromeric probes for chromosomes 1, 2, 3, 4, 10, 12, and 17 and whole-chromosome paint libraries for chromosomes 1, 2, 3, 4, 8, and 12, in conjunction with analysis of G-banded metaphases, allowed the major portion(s) of these 10 PPC-1 marker chromosomes to be defined. The results increase the number of identifiable chromosomal breakpoints in this cell line from 9 to 28 sites.

引用

收藏

页码：8 / &

相关论文

共 15 条

[1] CHROMOSOME STUDY OF 5 CANCERS OF THE PROSTATE [J].

ATKIN, NB ;

BAKER, MC .

HUMAN GENETICS, 1985, 70 (04) :359-364

[2] PHENOTYPIC AND CYTOGENETIC CHARACTERIZATION OF A CELL-LINE DERIVED FROM PRIMARY PROSTATIC-CARCINOMA [J].

BROTHMAN, AR ;

LESHO, LJ ;

SOMERS, KD ;

WRIGHT, GL ;

MERCHANT, DJ .

INTERNATIONAL JOURNAL OF CANCER, 1989, 44 (05) :898-903

[3]

BROTHMAN AR, 1990, CANCER RES, V50, P3795

[4] DETECTION OF CHROMOSOME-ABERRATIONS IN METAPHASE AND INTERPHASE TUMOR-CELLS BY INSITU HYBRIDIZATION USING CHROMOSOME-SPECIFIC LIBRARY PROBES [J].

CREMER, T ;

LICHTER, P ;

BORDEN, J ;

WARD, DC ;

MANUELIDIS, L .

HUMAN GENETICS, 1988, 80 (03) :235-246

[5] A SIMPLE METHOD OF REDUCING THE FADING OF IMMUNOFLUORESCENCE DURING MICROSCOPY [J].

JOHNSON, GD ;

ARAUJO, GMDN .

JOURNAL OF IMMUNOLOGICAL METHODS, 1981, 43 (03) :349-350

[6] PAINTING OF DEFINED CHROMOSOMAL REGIONS BY INSITU SUPPRESSION HYBRIDIZATION OF LIBRARIES FROM LASER-MICRODISSECTED CHROMOSOMES [J].

LENGAUER, C ;

ECKELT, A ;

WEITH, A ;

ENDLICH, N ;

PONELIES, N ;

LICHTER, P ;

GREULICH, KO ;

CREMER, T .

CYTOGENETICS AND CELL GENETICS, 1991, 56 (01) :27-30

[7] REPORT OF THE COMMITTEE-ON-CHROMOSOME-CHANGES-IN-NEOPLASIA [J].

MITELMAN, F ;

KANEKO, Y ;

TRENT, JM .

CYTOGENETICS AND CELL GENETICS, 1990, 55 (1-4) :358-386

[8] A HIGHLY CONSERVED REPETITIVE DNA-SEQUENCE, (TTAGGG)N, PRESENT AT THE TELOMERES OF HUMAN-CHROMOSOMES [J].

MOYZIS, RK ;

BUCKINGHAM, JM ;

CRAM, LS ;

DANI, M ;

DEAVEN, LL ;

JONES, MD ;

MEYNE, J ;

RATLIFF, RL ;

WU, JR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (18) :6622-6626

[9] CYTOGENETIC ANALYSIS USING QUANTITATIVE, HIGH-SENSITIVITY, FLUORESCENCE HYBRIDIZATION [J].

PINKEL, D ;

STRAUME, T ;

GRAY, JW .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (09) :2934-2938

[10] FLUORESCENCE INSITU HYBRIDIZATION WITH HUMAN CHROMOSOME-SPECIFIC LIBRARIES - DETECTION OF TRISOMY-21 AND TRANSLOCATIONS OF CHROMOSOME-4 [J].

PINKEL, D ;

LANDEGENT, J ;

COLLINS, C ;

FUSCOE, J ;

SEGRAVES, R ;

LUCAS, J ;

GRAY, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (23) :9138-9142