A GENERAL-METHOD FOR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF PYRROLIZIDINE ALKALOID FREE BASES AND N-OXIDES

被引：19

作者：

BROWN, MS

MOLYNEUX, RJ

ROITMAN, JN

机构：

[1] Western Regional Research Center, Agricultural Research Service, Usda, Albany, California, 94710

来源：

PHYTOCHEMICAL ANALYSIS | 1994年 / 5卷 / 05期

关键词：

HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; PYRROLIZIDINE ALKALOIDS; RADIAL COMPRESSION COLUMN;

D O I：

10.1002/pca.2800050507

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A reverse phase, ion-pair high performance liquid chromatographic method for the separation of five distinct structural subclasses of pyrrolizidine alkaloids has been developed. The system consists of a muBondapak CN or Nova-Pak Phenyl column in a radial compression apparatus, eluted isocratically with methanol: water (27:73) containing 0.01 M dibutylammonium phosphate. Symmetrical, well-resolved peaks were obtained for 30 pyrrolizidine alkaloids within a 15 min elution time. Both the free base and the N-oxide forms of the alkaloids can be analysed under the same conditions. The elution times are indicative of the relative polarities of the alkaloids being separated.

引用

页码：251 / 255

页数：5

共 21 条

[1] SEPARATION OF IONIC COMPOUNDS BY REVERSED-PHASE LIQUID-CHROMATOGRAPHY - AN UPDATE OF ION-PAIRING TECHNIQUES [J].