A CONVENIENT HIGH-YIELD SYNTHESIS OF N-4-ISOBUTYRYL-2'-O-METHYLCYTIDINE AND ITS MONOMER UNITS FOR INCORPORATION INTO OLIGONUCLEOTIDES

被引：5

作者：

VAGHEFI, MM

HOGREFE, RI

机构：

[1] GENTA Inc., San Diego, California, 92121

来源：

NUCLEOSIDES & NUCLEOTIDES | 1993年 / 12卷 / 10期

关键词：

D O I：

10.1080/07328319308016200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We designed an efficient three step procedure for the synthesis of N-4-isobutyryl-2'-O-methylcytidine. This protected nucleoside was then used to prepare a methylphosphonamidite monomer for incorporation into oligonucleotides. Transamination at the C-4 position of cytidine using ethylenediamine, which has been reported for the N-4-benzoyl cytidine, was not observed with N-4-isobutyryl protected 2'-O-methylcytidine

引用

页码：1007 / 1013

页数：7

共 9 条

[1]

HEIKKILA J, 1982, CHEM SCRIPTA, V20, P251

[2] DEPROTECTION OF METHYLPHOSPHONATE OLIGONUCLEOTIDES USING A NOVEL ONE-POT PROCEDURE [J].