REQUIREMENT FOR THE MAP KINASE KINASE MAP KINASE CASCADE IN XENOPUS-OOCYTE MATURATION

被引:200
作者
KOSAKO, H
GOTOH, Y
NISHIDA, E
机构
[1] KYOTO UNIV, INST VIRUS RES, DEPT MOLEC BIOL & GENET, SAKYO KU, KYOTO 60601, JAPAN
[2] UNIV TOKYO, FAC SCI, DEPT BIOPHYS & BIOCHEM, TOKYO 113, JAPAN
关键词
KINASE CASCADE; MAP KINASE KINASE; OOCYTE MATURATION; SIGNAL TRANSDUCTION; XENOPUS;
D O I
10.1002/j.1460-2075.1994.tb06489.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
MAP kinase kinase (MAPKK) has been identified as a protein factor that can induce phosphorylation and activation of inactive MAP kinase in vitro. In this study, we produced an anti-Xenopus MAPKK antibody that can specifically inhibit Xenopus MAPKK activity in vitro. Microinjection of this antibody into immature oocytes prevented progesterone-induced MAP kinase activation. Moreover, progesterone-induced histone H1 kinase activation and germinal vesicle breakdown (GVBD) were inhibited in the oocytes injected previously with this antibody. Furthermore, when a bacterially expressed Mos was introduced into immature oocytes, Mos-induced MAP kinase activation and GVBD were blocked in the oocytes injected with the anti-MAPKK antibody. These results show that MAPKK is responsible for the activation of MAP kinase in vivo and that the MAPKK/MAP kinase cascade plays a pivotal role in the MPF activation during the oocyte maturation process.
引用
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页码:2131 / 2138
页数:8
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