MOLECULAR ANALYSIS OF MERCURY-RESISTANT BACILLUS ISOLATES FROM SEDIMENT OF MINAMATA BAY, JAPAN

被引：52

作者：

NAKAMURA, K ^{[1
]}

SILVER, S ^{[1
]}

机构：

[1] UNIV ILLINOIS,COLL MED,DEPT MICROBIOL & IMMUNOL,CHICAGO,IL 60612

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1994年 / 60卷 / 12期

关键词：

D O I：

10.1128/AEM.60.12.4596-4599.1994

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Bacillus isolates from Minamata Bay sediment were selected for the ability to volatilize mercury from a range of organomercurials (including methylmercury). Chromosomal DNA from 74 of 78 such strains hybridized with the mer mercury resistance operon DNA from marine Bacillus sp. strain RC607 (Y. Wang, M. Moore, H. S. Levinson, S. Silver, C. Walsh, and I. Mahler, J. Bacteriol. 171:83-92, 1989). The most frequent classes with regard to restriction nuclease site maps of the mer operon for the new isolates were identical to or closely related to the mer determinant of strain RC607. PCR amplification analysis with primers designed from the strain RC607 mer operon gave products of precisely the predicted size with the 74 Minamata Bay isolates.

引用

页码：4596 / 4599

页数：4

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