SOME PROPERTIES OF A LIPID MOBILIZING SUBSTANCE FROM CALF MID-BRAIN

1. 1. A substance with lipid mobilizing properties has been isolated from the calf mid-brain. 2. 2. The lipid mobilizing factor was chromatographically, electrophoretically and spectroscopically similar to hypoxanthine, but differed from the purine in solubility and in lipolytic properties. 3. 3. The incubation medium retained lipid mobilizing properties following tissue exposure, and the tissue retained some lipolytic activity after exposure to the lipid mobilizing factor. 4. 4. A parallelism existed between the relationship of the mid-brain lipid mobilizing factor and adrenergic blocking agents, and epinephrine and adrenergic blocking agents with respect to lipid mobilization. 5. 5. An inhibitor of lipid mobilization also was present in the crude mid-brain extract. The inhibitor, after separation from the lipid mobilizing component, suppressed the lipolytic activities of the purified mid-brain extract and the catecholamines (epinephrine and norepinephrine). © 1969.