STUDIES ON CELL MOTILITY IN INFLAMMATION .1. CHEMOTACTIC ACTIVITY OF EXPERIMENTAL, IMMUNOLOGICAL AND NON-IMMUNOLOGICAL, INFLAMMATORY EXUDATES

The accumulation of leucocytes at the site of inflammation may be brought about by chemotaxis or proliferation in the extravascular tissues. The present paper focuses on the chemotactic properties of different types of experimental inflammatory pleural exudates, using a modified Boyden chamber. The time-course of carrageenan-induced exudate chemotactic activity for polymorphs was maximat at 4 h, thereafter diminishing towards 24 and 48 h. Chemotactic activity for mononuclear cells remained unchanged throughout the 4-48 h time-course. Heating the exudates to 56°C for 1 h partially reduced chemotactic activity. These results correlate well with the migration of polymorph and mononuclear cells into the pleural cavity during carrageenan-induced pleurisy. The potency of polymorph and mononuclear cell chemotactic activity of different exudates was of the following order: carrageenan > calcium pyrophosphate > reverse passive Arthus > dextran. These results are discussed in order to elucidate the differences between the underlying mechanisms responsible for leucocyte accumulation in different types of inflammatory reaction. © 1979 Birkhäuser-Verlag.