The crystalline components responsible for the room temperature fluorescence, low temperature phosphorescence, and low temperature fluorescence of expressed lime oil have been isolated and identified. Two of the eleven components have not previously been isolated from expressed lime oil. Luminescence characteristics for the individual components are compared with those for samples of expressed lime oil of different geographical origin. The coumarin and psoralen derivatives responsible for the various emission characteristics of the whole oil are identified. © 1969, American Chemical Society. All rights reserved.