DIRECT KINETIC ASSAY FOR MANDELATE RACEMASE USING CIRCULAR DICHROIC MEASUREMENTS

被引：39

作者：

SHARP, TR

HEGEMAN, GD

KENYON, GL

机构：

[1] UNIV CALIF SAN FRANCISCO,DEPT PHARMACEUT CHEM,SAN FRANCISCO,CA 94143

[2] UNIV CALIF SAN FRANCISCO,CARDIOVASC RES INST,SAN FRANCISCO,CA 94143

[3] INDIANA UNIV,DEPT CHEM,BLOOMINGTON,IN 47401

[4] INDIANA UNIV,DEPT BIOL,BLOOMINGTON,IN 47401

[5] UNIV CALIF SAN FRANCISCO,DEPT BIOCHEM & BIOPHYS,SAN FRANCISCO,CA 94143

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 94卷 / 02期

关键词：

D O I：

10.1016/0003-2697(79)90368-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new, direct kinetic assay for mandelate racemase has been developed. The assay depends upon changes with time in circular dichroic ellipticities of mandelate solutions at 262 nm. The technique should allow more precise kinetic measurements of the mandelate racemase reaction than have heretofore been possible. Also, the approach to equilibrium can be measured from either (R)- or (S)-mandelate with equal facility. The technique, should, moreover, be extendible to other enzyme-catalyzed reactions involving changes with time in circular dichroic ellipticities. © 1979.

引用

页码：329 / 334

页数：6

共 15 条

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