TRANSCRIPT MAPPING OF ABUTILON MOSAIC-VIRUS, A GEMINIVIRUS

被引：50

作者：

FRISCHMUTH, S ^{[1
]}

FRISCHMUTH, T ^{[1
]}

JESKE, H ^{[1
]}

机构：

[1] INST ALLGEMEINE BOT,OHNHORSTSTR 18,W-2000 HAMBURG 52,GERMANY

来源：

VIROLOGY | 1991年 / 185卷 / 02期

关键词：

D O I：

10.1016/0042-6822(91)90530-O

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The transcripts of the DNA of Abutilon mosaic virus, a geminivirus with a bipartite genome (DNA A and DNA B), were characterized by Northern blot hybridization, S1 nuclease assay, primer extension analysis, and sequencing of the 3′ termini of cDNA clones. It was shown that transcription is bidirectional and that the transcripts are polyadenylated. Two overlapping transcripts of 1.6 and 0.7 kb were mapped to the complementary strand of DNA A and two of 1.3 and 1.2 kb to the complementary strand of DNA B. One transcript of 0.9 kb was mapped to the viral sense in DNA A and one of 1.0 kb to the viral sense in DNA B. The ends of complementary and viral mRNA overlapped in both genome parts in regions rich in polyadenylation signals. © 1991.

引用

页码：596 / 604

页数：9

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