EXPRESSION OF A BRASSICA-NAPUS EXTENSIN GENE IN THE VASCULAR SYSTEM OF TRANSGENIC TOBACCO AND RAPE PLANTS

被引：27

作者：

SHIRSAT, AH ^{[1
]}

WILFORD, N ^{[1
]}

EVANS, IM ^{[1
]}

GATEHOUSE, LN ^{[1
]}

CROY, RRD ^{[1
]}

机构：

[1] UNIV DURHAM, DEPT BIOL SCI, PLANT MOLEC BIOL GRP, DURHAM DH1 3LE, ENGLAND

来源：

PLANT MOLECULAR BIOLOGY | 1991年 / 17卷 / 04期

关键词：

BRASSICA-NAPUS; EXTENSIN GENES; GUS GENE FUSIONS; PHLOEM-SPECIFIC EXPRESSION; TRANSGENIC PLANTS;

D O I：

10.1007/BF00037055

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The organs and tissues where the Brassica napus extA extension gene is expressed have been identified. The extA gene with 3.75 kb of 5' flanking sequence was transferred to tobacco via disarmed Agrobacterium tumefaciens vectors and transgenic plants regenerated. The gene was found to be inactive in transgenic tobacco leaf, but was active as measured by RNA transcript assays in both stem and root tissues. To determine the cell-specific expression pattern of the extA gene, a promoter-reporter gene fusion construct was made consisting of 1.0 kb of 5' extA sequence fused to the coding region of the glucuronidase (GUS) gene. This fusion construct was introduced into B. napus via Agrobacterium rhizogenes, and expression of GUS in transgenic rape hairy roots was examined. GUS activity was only seen in the vascular tissues of the rape root, and was found to be specifically localised in the phloem.

引用

页码：701 / 709

页数：9

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