INTERSPECIES VARIATION OF SCHISTOSOME 28-KDA GLUTATHIONE S-TRANSFERASES

被引：88

作者：

TROTTEIN, F

GODIN, C

PIERCE, RJ

SELLIN, B

TAYLOR, MG

GORILLOT, I

SILVA, MS

LECOCQ, JP

CAPRON, A

机构：

[1] INST NACL SAUDE,OPORTO,PORTUGAL

[2] TRANSGENE SA,STRASBOURG,FRANCE

[3] CTR RECH MENINGITES & SCHISTOSOMIASES,NIAMEY,NIGER

[4] UNIV LONDON LONDON SCH HYG & TROP MED,LONDON WC1E 7HT,ENGLAND

来源：

MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 1992年 / 54卷 / 01期

关键词：

SCHISTOSOME; GLUTATHIONE S-TRANSFERASE; MOLECULAR CLONING; B-CELL EPITOPE; VACCINE DEVELOPMENT;

D O I：

10.1016/0166-6851(92)90095-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The 28-kDa glutathione S-transferase from Schistosoma mansoni (Sm28GST) is a candidate vaccine antigen. To evaluate the antigenic and phylogenetic variations between the 28-kDa GSTs from 4 species of schistosome, we have cloned and sequenced the 28-kDa GSTs from Schistosoma haematobium (Sh28GST) and Schistosoma bovis (Sb28GST). Sb28GST and Sh28GST are more similar to each other (97%) than to Sm28GST (90%) and particularly to the 28-kDa GST from Schistosoma japonicum (Sj28GST, 77%). Antisera directed against the major Sm28GST epitopes revealed differences in the recognition of the 28-kDa GSTs from the other schistosome species suggesting that these regions have been subjected to evolutionary pressure. The consequences of such species-specific epitopes on the development of a multi-species anti-schistosome vaccine are discussed.

引用

页码：63 / 72

页数：10

共 38 条

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