H+/GLYCYL-GLYCINE COTRANSPORT IN EEL INTESTINAL BRUSH-BORDER MEMBRANE-VESICLES - STUDIES WITH THE PH-SENSITIVE DYE ACRIDINE-ORANGE

被引：21

作者：

VERRI, T

MAFFIA, M

STORELLI, C

机构：

[1] Laboratorio di Fisiologia, Dipartimento di Biologia, Università di Lecce, Lecce

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1992年 / 1110卷 / 01期

关键词：

FLUORESCENCE; ACRIDINE ORANGE; PROTON DIPEPTIDE COTRANSPORT; INTESTINE; (EEL);

D O I：

10.1016/0005-2736(92)90303-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Monitoring the fluorescence quenching of the pH-sensitive dye Acridine orange, proton accumulation in the presence of an inside-negative transmembrane potential was measured in eel (Anguilla anguilla) intestinal brush-border membrane vesicles. It was demonstrated that the proton accumulation was specifically increased by the presence of the dipeptide glycyl-glycine in the extravesicular space, showing saturation kinetics at increasing dipeptide concentrations and was specifically inhibited by diethylpyrocarbonate. Data reported suggest the presence of an electrical-potential-dependent H+/glycyl-glycine cotransport system in the eel intestinal brush-border membrane vesicles.

引用

页码：123 / 126

页数：4

共 8 条

[1] IS INTESTINAL PEPTIDE-TRANSPORT ENERGIZED BY A PROTON GRADIENT [J].