SUPERFICIALLY POROUS SILICA MICROSPHERES FOR THE FAST HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF MACROMOLECULES

被引：165

作者：

KIRKLAND, JJ ^{[1
]}

机构：

[1] DUPONT CO,CENT RES & DEV,EXPTL STN,WILMINGTON,DE 19880

来源：

ANALYTICAL CHEMISTRY | 1992年 / 64卷 / 11期

关键词：

D O I：

10.1021/ac00035a009

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

New superficially porous "Poroshell" particles have been synthesized and tested for HPLC separations of macromolecules. Spray-drying techniques were used to prepare silica microspheres that typically have a 5-mu-m solid core and a approximately 1-mu-m-thick outer shell with 300-angstrom pores. Compared to conventional totally porous particles of the same size, Poroshell particles show improved mass-transfer kinetic properties that permit more rapid separations of macromolecules. The utility of the new Poroshell column packing is demonstrated with separations of proteins and synthetic polymers.

引用

页码：1239 / 1245

页数：7

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