ESCHERICHIA-COLI SINGLE-STRANDED DNA-BINDING PROTEIN ALTERS THE STRUCTURE OF INTRAMOLECULAR TRIPLEXES IN PLASMIDS

被引：6

作者：

KLYSIK, J

SHIMIZU, M

机构：

[1] TEXAS A&M UNIV SYST,TEXAS MED CTR,INST BIOSCI & TECHNOL,HOUSTON,TX 77030

[2] UNIV ALABAMA,SCH DENT,BIRMINGHAM,AL 35294

来源：

FEBS LETTERS | 1993年 / 333卷 / 03期

关键词：

TRIPLE-STRANDED DNA; SUPERCOILING; SSB PROTEIN; CHEMICAL PROBE; UNPAIRED BASE;

D O I：

10.1016/0014-5793(93)80666-I

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The ability of the Escherichia coli single-stranded DNA-binding protein (SSB) to recognize structural features associated with intramolecular triplex formation in oligopurine . oligopyrimidine (pur . pyr) inserts in recombinant plasmids was evaluated. The SSB protein binds to supercoiled plasmids and causes a site-preferential increase in OsO4 reactivity of the pyrimidine strand involved in the formation of the Hy-3 isomer of the triplex structure. The E. coli RecA protein showed no reaction with triplexes in similar studies. This behavior is consistent with SSB-mediated unpairing of the H-DNA-forming region.

引用

页码：261 / 267

页数：7

共 50 条

[1] SYNTHESIS OF RECA PROTEIN AND INDUCTION OF BACTERIOPHAGE-LAMBDA IN SINGLE-STRAND DEOXYRIBONUCLEIC ACID-BINDING PROTEIN MUTANTS OF ESCHERICHIA-COLI [J].

BALUCH, J ;

CHASE, JW ;

SUSSMAN, R .

JOURNAL OF BACTERIOLOGY, 1980, 144 (02) :489-498

[2] FORMATION OF DNA TRIPLEXES ACCOUNTS FOR ARRESTS OF DNA-SYNTHESIS AT D(TC)N AND D(GA)N TRACTS [J].

BARAN, N ;

LAPIDOT, A ;

MANOR, H .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (02) :507-511

[3]

BLAHO JA, 1988, J BIOL CHEM, V263, P14446

[4] NEGATIVE CO-OPERATIVITY IN ESCHERICHIA-COLI SINGLE-STRAND BINDING PROTEIN-OLIGONUCLEOTIDE INTERACTIONS .1. EVIDENCE AND A QUANTITATIVE MODEL [J].

BUJALOWSKI, W ;

LOHMAN, TM .

JOURNAL OF MOLECULAR BIOLOGY, 1989, 207 (01) :249-268

[5]

CHASE JW, 1986, ANNU REV BIOCHEM, V55, P103, DOI 10.1146/annurev.bi.55.070186.000535

[6] THE ROLE OF ESCHERICHIA-COLI SINGLE-STRANDED-DNA BINDING-PROTEIN IN DNA METABOLISM [J].

CHASE, JW .

BIOESSAYS, 1984, 1 (05) :218-222

[7] ESCHERICHIA-COLI SINGLE-STRAND BINDING-PROTEIN ORGANIZES SINGLE-STRANDED-DNA IN NUCLEOSOME-LIKE UNITS [J].

CHRYSOGELOS, S ;

GRIFFITH, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (19) :5803-5807

[8]

COLLIER DA, 1988, J BIOL CHEM, V263, P7397

[9]

GALAZKA G, 1986, J BIOL CHEM, V261, P7093

[10] MUTANT SINGLE-STRAND BINDING-PROTEIN OF ESCHERICHIA-COLI - GENETIC AND PHYSIOLOGICAL CHARACTERIZATION [J].

GLASSBERG, J ;

MEYER, RR ;

KORNBERG, A .

JOURNAL OF BACTERIOLOGY, 1979, 140 (01) :14-19

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