HETEROLOGOUS AND HOMOLOGOUS PROTECTION AGAINST INFLUENZA-A BY DNA VACCINATION - OPTIMIZATION OF DNA VECTORS

被引：224

作者：

MONTGOMERY, DL ^{[1
]}

SHIVER, JW ^{[1
]}

LEANDER, KR ^{[1
]}

PERRY, HC ^{[1
]}

FRIEDMAN, A ^{[1
]}

MARTINEZ, D ^{[1
]}

ULMER, JB ^{[1
]}

DONNELLY, JJ ^{[1
]}

LIU, MA ^{[1
]}

机构：

[1] MERCK RES LABS,DEPT VIRUS & CELL BIOL,SUNNEYTOWN PIKE WP16 101,W POINT,PA 19486

来源：

DNA AND CELL BIOLOGY | 1993年 / 12卷 / 09期

关键词：

D O I：

10.1089/dna.1993.12.777

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have recently shown that direct injection of DNA can be an effective vaccine strategy eliciting both humoral and cell-mediated immune responses. Vectors were designed specifically for vaccination by direct DNA injection and refined to improve plasmid production in Escherichia coli. The vectors consist of a pUC-19 backbone with the cytomegalovirus (CMV) IE1 enhancer, promoter, and intron A transcription regulatory elements and the BGH polyadenylation sequences driving the expression of the reporter gene CAT or influenza A nucleoprotein (NP) or hemagglutinin (HA). The respective vectors expressed high levels of chloramphenicol acetyltransferase (CAT) and NP in tissue culture, and yielded 14-15 mg of purified plasmid per liter of Escherichia coli culture. Immunization of mice with the NP and HA expression vectors resulted in protection from subsequent lethal challenges of influenza using either heterologous or homologous strains, respectively.

引用

页码：777 / 783

页数：7

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