LOW-MOLECULAR-WEIGHT THERMOSTABLE BETA-D-GLUCOSIDASE FROM ACIDOTHERMUS-CELLULOLYTICUS

被引：5

作者：

ADNEY, WS ^{[1
]}

TUCKER, MP ^{[1
]}

NIEVES, RA ^{[1
]}

THOMAS, SR ^{[1
]}

HIMMEL, ME ^{[1
]}

机构：

[1] NATL RENEWABLE ENERGY LAB,DIV ALTERNAT FUELS,APPL BIOL SCI SECT,GOLDEN,CO 80401

来源：

BIOTECHNOLOGY LETTERS | 1995年 / 17卷 / 01期

关键词：

D O I：

10.1007/BF00134195

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Industrial use of cellulase enzymes, including beta-D-glucosidases, can be greatly improved and expanded by the discovery and characterization of more robust, thermotolerant enzymes. A new thermostable beta-D-glucosidase was isolated from Acidothermus cellulolyticus culture broth. This enzyme has a molecular weight of 52.5 kDa, an isoelectric pI of 4.1, and a pH activity optimum of 4.5. The optimum temperature for activity on p-nitrophenyl-beta-D-glucoside is 63 degrees C. This value is approximately 20 degrees C higher than temperature optima displayed by most previously characterized beta-D-glucosidases.

引用

页码：49 / 54

页数：6

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