PHAGE LAMBDA RECEPTOR CHROMOSOMES FOR DNA FRAGMENTS MADE WITH RESTRICTION ENDONUCLEASE-I OF BACILLUS-AMYLOLIQUEFACIENS-H

被引:19
作者
KLEIN, B
MURRAY, K
机构
[1] Department of Molecular Biology University of Edinburgh Kings Buildings Edinburgh
关键词
D O I
10.1016/0022-2836(79)90537-0
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A lambda derivative with DNA resistant to attack by R. BamHI has been obtained following mutagenesis to remove a single target for this enzyme. The two central targets for R. BamHI were then introduced into the genome of this phage and the DNA between them removed in vitro to give a lambda insertion vector for fragments of DNA produced by digestion with R. BamHI. © 1979.
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页码:289 / 294
页数:6
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