MANGANESE OXIDATION BY MICROBIAL CONSORTIA FROM SAND FILTERS

被引:57
作者
VANDENABEELE, J
DEBEER, D
GERMONPRE, R
VERSTRAETE, W
机构
[1] STATE UNIV GHENT,MICROBIAL ECOL LAB,COUPURE LINKS 653,B-9000 GENT,BELGIUM
[2] UNIV AMSTERDAM,DEPT CHEM TECHNOL,1018 WW AMSTERDAM,NETHERLANDS
[3] VLAAMSE MAATSCHAPPIJ WATERVOORZIENING,CTR WATER RES,B-1040 BRUSSELS,BELGIUM
关键词
D O I
10.1007/BF00171973
中图分类号
Q14 [生态学(生物生态学)];
学科分类号
071012 ; 0713 ;
摘要
The role of microbial consortia on the removal of manganese (Mn) was examined on sand from three different Belgian rapid sand filters for the treatment of ground water. Microorganisms closely associated with deposits of Fe and amorphous Mn precipitates were observed by SEM and EDAX techniques on sand from the filters able to remove Mn efficiently. Bacterial counts were performed. Of the CFU enumerated on PYM-medium, 25-33% displayed Mn-oxidizing activity. Batch cultures were set up by inoculating a Mn-containing, low organic medium with sand from one of the filters. Microbial growth resulted in the formation of Mn-removing bacterial flocs and a pH increase. Suppression of microbial growth by addition of azide, kanamycin, or by autoclaving reduced removal of Mn2+ from 0.5 mM/day to 0.05-0.11 mM/day. Buffering the pH of the medium at 7.5 (0.1 mM Hepes) decelerated the Mn removal but did not halt it, whereas microelectrode measurements revealed a clear pH drop of about 0.7 units inside bacterial flocs. In the absence of Mn2+, the pH drop was only 0.4 units. The auto-catalytic removal of Mn by the Mn oxide coated filter sand was not sufficient to explain the Mn removal observed. Inactivated cells were not capable of a pronounced autocatalytic Mn removal. Experiments with enrichment cultures indicated that the Mn-removing capacity of the microbial sand filter consortia was not constitutive but was promoted by preadaptation and the presence of a substratum. These results clearly link Mn oxidation in rapid sand filters to microbial processes.
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页码:91 / 108
页数:18
相关论文
共 37 条
[1]  
BEVERIDGE TJ, 1984, CURRENT PERSPECTIVES, P601
[2]  
Boudou J.P., 1985, WATER SUPPLY, V3, P151
[3]   COLORIMETRIC DETERMINATION OF MANGANESE IN ANOXIC WATERS [J].
BREWER, PG ;
SPENCER, DW .
LIMNOLOGY AND OCEANOGRAPHY, 1971, 16 (01) :107-&
[4]  
Chr Czekalla, 1985, WATER SUPPLY, V3, P111
[5]   CATALYTIC-OXIDATION OF AQUEOUS MN(II) [J].
COUGHLIN, RW ;
MATSUI, I .
JOURNAL OF CATALYSIS, 1976, 41 (01) :108-123
[6]  
CULLIMORE DR, 1987, 39TH P ANN CONV W CZ, P177
[7]   GRADIENTS IN IMMOBILIZED BIOLOGICAL-SYSTEMS [J].
DEBEER, D ;
VANDENHEUVEL, JC .
ANALYTICA CHIMICA ACTA, 1988, 213 (1-2) :259-265
[8]   ENHANCED BIODEGRADATION OF O-CRESOL BY ACTIVATED-SLUDGE IN THE PRESENCE OF POWDERED ACTIVATED CARBON [J].
DEJONGE, RJ ;
BREURE, AM ;
VANANDEL, JG .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1991, 34 (05) :683-687
[9]   IS DISSOLVED MN-2+ BEING OXIDIZED BY O-2 IN ABSENCE OF MN-BACTERIA OR SURFACE CATALYSTS [J].
DIEM, D ;
STUMM, W .
GEOCHIMICA ET COSMOCHIMICA ACTA, 1984, 48 (07) :1571-1573
[10]  
EHRLICH HL, 1990, ARCH MICROBIOL, V154, P12