A SIMPLE AND EFFICIENT SYSTEM FOR THE CONSTRUCTION OF PHOA GENE FUSIONS IN GRAM-NEGATIVE BACTERIA

被引：4

作者：

DUCHENE, AM ^{[1
]}

PATTE, J ^{[1
]}

GUTIERREZ, C ^{[1
]}

CHANDLER, M ^{[1
]}

机构：

[1] CNRS,MOLEC GENET & MICROBIOL UNIT,118 ROUTE NARBONNE,F-31062 TOULOUSE,FRANCE

来源：

GENE | 1992年 / 114卷 / 01期

关键词：

ENVELOPE PROTEINS; ALKALINE PHOSPHATASE; BETA-LACTAMASE; TRANSPOSON; PLASMID;

D O I：

10.1016/0378-1119(92)90714-Z

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have developed a two-plasmid system for generating gene fusions between phoA and cloned genes encoding envelope proteins. The vector plasmid carries a temperature-sensitive replication system and can be rescued at high temperature by insertion of an IS1-based transposon carrying the ori region of pBR322 and a phoA gene lacking transcription and translation initiation signals. The vector plasmid also carries the transfer origin of the conjugative plasmid, F, permitting transfer into a suitable recipient strain. We have used this system in the analysis of the bla gene cloned from pBR322.

引用

页码：103 / 107

页数：5

共 26 条

[1] DETERMINANTS OF MEMBRANE-PROTEIN TOPOLOGY [J].

BOYD, D ;

MANOIL, C ;

BECKWITH, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (23) :8525-8529

[2]

Boyd D, 1987, PHOSPHATE METABOLISM, P89

[3] MINI-TN5 TRANSPOSON DERIVATIVES FOR INSERTION MUTAGENESIS, PROMOTER PROBING, AND CHROMOSOMAL INSERTION OF CLONED DNA IN GRAM-NEGATIVE EUBACTERIA [J].

DELORENZO, V ;

HERRERO, M ;

JAKUBZIK, U ;

TIMMIS, KN .

JOURNAL OF BACTERIOLOGY, 1990, 172 (11) :6568-6572

[4] OMEGON-KM - A TRANSPOSABLE ELEMENT DESIGNED FOR INVIVO INSERTIONAL MUTAGENESIS AND CLONING OF GENES IN GRAM-NEGATIVE BACTERIA [J].

FELLAY, R ;

KRISCH, HM ;

PRENTKI, P ;

FREY, J .

GENE, 1989, 76 (02) :215-226

[5] A SYSTEM FOR INVIVO SELECTION OF GENOMIC REARRANGEMENTS WITH PREDETERMINED END-POINTS IN ESCHERICHIA-COLI USING MODIFIED TN10 TRANSPOSONS [J].

FRANCOIS, V ;

LOUARN, J ;

PATTE, J ;

LOUARN, JM .

GENE, 1987, 56 (01) :99-108

[6] SEQUENCE-ANALYSIS OF TN9 INSERTIONS IN THE LACZ GENE [J].

GALAS, DJ ;

CALOS, MP ;

MILLER, JH .

JOURNAL OF MOLECULAR BIOLOGY, 1980, 144 (01) :19-41

[7] REPLICATION OF PSC101 - EFFECTS OF MUTATIONS IN THE ESCHERICHIA-COLI DNA-BINDING PROTEIN IHF [J].

GAMAS, P ;

BURGER, AC ;

CHURCHWARD, G ;

CARO, L ;

GALAS, D ;

CHANDLER, M .

MOLECULAR & GENERAL GENETICS, 1986, 204 (01) :85-89

[8] OSMOTIC INDUCTION OF GENE OSMC EXPRESSION IN ESCHERICHIA-COLI K12 [J].

GUTIERREZ, C ;

DEVEDJIAN, JC .

JOURNAL OF MOLECULAR BIOLOGY, 1991, 220 (04) :959-973

[9] A PLASMID FACILITATING INVITRO CONSTRUCTION OF PHOA-GENE FUSIONS IN ESCHERICHIA-COLI [J].

GUTIERREZ, C ;

DEVEDJIAN, JC .

NUCLEIC ACIDS RESEARCH, 1989, 17 (10) :3999-3999

[10] MUTATIONS TO TEMPERATURE SENSITIVITY IN R-PLASMID PSC101 [J].

HASHIMOTOGOTOH, T ;

SEKIGUCHI, M .

JOURNAL OF BACTERIOLOGY, 1977, 131 (02) :405-412

← 1 2 3 →