STUDIES ON IN VITRO RNA SYNTHESIS BY CHROMATIN FRACTIONS FROM RAT LIVER AND NOVIKOFF HEPATOMA

Eu- and heterochromatin fractions prepared from normal liver and Novikoff hepatoma were tested for their capacity to direct the synthesis of RNA. Using purified RNA polymerase, template activity was measured by the incorporation of C14 GTP into RNA. Unlike DNA, chromatin fractions had increased template activity at increased salt concentrations. No difference was observed in the priming capacity of either eu- or heterochromatin fractions derived from normal liver or Novikoff hepatoma. © 1969.