POTENT INHIBITION OF THYMIDYLATE SYNTHASE BY 2 SERIES OF NONCLASSICAL QUINAZOLINES

被引:57
作者
MCNAMARA, DJ [1 ]
BERMAN, EM [1 ]
FRY, DW [1 ]
WERBEL, LM [1 ]
机构
[1] WARNER LAMBERT PARKE DAVIS, DEPT BIOCHEM, DIV RES, ANN ARBOR, MI 48105 USA
关键词
D O I
10.1021/jm00169a040
中图分类号
R914 [药物化学];
学科分类号
100701 ;
摘要
The synthesis and biological activity of two series of nonclassical thymidylate synthase (TS) inhibitors are described. The first is a series of 10-propargyl-5,8-dideazafolic acid derivatives (l0a-j) and the second is a series of the analogous 2-desamino derivatives (13a-c,k), both bearing a more lipophilic substituent on the phenyl ring than the CO-glutamate of classical antifolates. Compounds l0a-j were prepared in a straightforward manner, generally by treatment of N-[6-(bromomethyl)-3,4-dihydro-4-oxo-2-quinazolinyl]-2,2-dimethylpropanamide (6) with various phenyl-substituted N-propargylanilines (8), followed by deprotection. Compounds 13a-c,k were prepared similarly from [6-(bromomethyl)-4-oxo-3(4/f)-quinazolinyl]methyl 2,2-dimethylpropanoate (11). The compounds were tested for inhibition of purified L1210 TS and for inhibition of L1210 cell growth in vitro. Several of these nonclassical analogues approached the TS inhibitory potency of 10-propargyl-5,8-dideazafolic acid (1, CB3717), a glutamate-containing TS inhibitor. 2-Amino target compounds 10a-j were generally potent inhibitors of L1210 TS, with IC50S within the range of 0.51-11.5 μm,compared to 0.05 μM for 1. The order of potency for phenyl substitution at the 4-position in this series was the following: COCF3≥ N02≥ CONH2≥ COCH3= SO2NMe2 = CN » OCF3≥ F. The 2-desamino target compounds 13a-c,k also exhibited significant, although diminished, TS inhibition. Both series were growth inhibitory to cells in tissue culture and this inhibition could be reversed by thymidine alone, indicating that the primary target was TS. None of the compounds was a potent inhibitor of dihydrofolate reductase. These studies indicate that the presence of the glutamate moiety in folate analogues is not an absolute requirement for potent inhibition of TS. © 1990, American Chemical Society. All rights reserved.
引用
收藏
页码:2045 / 2051
页数:7
相关论文
共 32 条
  • [1] NEW SYNTHETIC ROUTE TO QUINAZOLINE ANALOGS OF FOLIC-ACID
    ACHARYA, SP
    HYNES, JB
    [J]. JOURNAL OF HETEROCYCLIC CHEMISTRY, 1975, 12 (06) : 1283 - 1286
  • [2] BEARDSLEY G P, 1986, P953
  • [3] Calvert A H, 1987, NCI Monogr, P213
  • [4] A PHASE-I EVALUATION OF THE QUINAZOLINE ANTIFOLATE THYMIDYLATE SYNTHASE INHIBITOR, N-10-PROPARGYL-5,8-DIDEAZAFOLIC ACID, CB3717
    CALVERT, AH
    ALISON, DL
    HARLAND, SJ
    ROBINSON, BA
    JACKMAN, AL
    JONES, TR
    NEWELL, DR
    SIDDIK, ZH
    WILTSHAW, E
    MCELWAIN, TJ
    SMITH, IE
    HARRAP, KR
    [J]. JOURNAL OF CLINICAL ONCOLOGY, 1986, 4 (08) : 1245 - 1252
  • [5] CALVERT AH, 1980, HUMAN CANCER ITS CHA, P272
  • [6] CHENG YC, 1985, CANCER RES, V45, P598
  • [7] BIOLOGICAL AND BIOCHEMICAL-PROPERTIES OF NEW ANTICANCER FOLATE ANTAGONISTS
    FRY, DW
    JACKSON, RC
    [J]. CANCER AND METASTASIS REVIEWS, 1987, 5 (03) : 251 - 270
  • [8] FRY DW, 1989, P AM ASSOC CANC RES, P30
  • [9] GARITO AF, 1980, MAKROMOL CHEM, V181, P1605
  • [10] Hantzsch A, 1901, LIEBIGS ANN CHEM, V314, P339