LYMPHOCYTE-B AND LYMPHOCYTE-T COLONY FORMATION IN AGAR - 2-MERCAPTOETHANOL-ACTIVATED ALBUMIN CAN SUBSTITUTE FOR 2-MERCAPTOETHANOL

被引：18

作者：

CLAESSON, MH

FLAD, HD

OPITZ, HG

机构：

[1] UNIV ULM,DEPT MICROBIOL,IMMUNOL LAB,D-7900 ULM,FED REP GER

[2] BAYER AG,INST IMMUNOL & ONCOL,D-5600 WUPPERTAL 1,FED REP GER

来源：

CELLULAR IMMUNOLOGY | 1979年 / 46卷 / 02期

关键词：

D O I：

10.1016/0008-8749(79)90426-X

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The effect of 2-mercaptoethanol (2-ME) activated albumin (MaSF) on mouse B lymphocyte colony (BLC) formation and on human phytohemagglutinin (PHA)-induced T lymphocyte colony formation (TLC) formation in semisolid agar medium was studied. MaSF was found to stimulate colony formation comparable to the stimulation obtained with 2-ME. MaSF could not substitute for serum in any of the agar culture systems. BLC formation stimulated by MaSF was obtained with spleen cells from athymic nude mice and nonadherent spleen and lymph node cells of normal mice suggesting a direct effect of MaSF on the colony-forming B cell without interference with T cells or macrophages. The results suggest that the stimulatory effect of 2-ME in the BLC and TLC agar systems is mediated by 2-ME-activated albumin present in the culture medium. © 1979.

引用

页码：398 / 404

页数：7

共 18 条

[1] PROMOTION OF REPLICATION IN LYMPHOID-CELLS BY SPECIFIC THIOLS AND DISULFIDES IN-VITRO - EFFECTS ON MOUSE LYMPHOMA CELLS IN COMPARISON WITH SPLENIC LYMPHOCYTES [J].